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Poly-histidine tags (His-tags) are among the most commonly used affinity tags.
We used affinity column chromatography to identify rice RACK1 as an interactor with OsRac1 (Nakashima et al. 2008) (Fig. 4).
The enriched saccharides together with the PMNPs can be directly detected by MALDI-TOF MS, without further eluting steps, different from currently widely used affinity columns.
During the development of purification protocols for rh.10, hu.37, AAV8, rh.64R1, AAV3B, and AAV9 vectors, based on a widely used affinity resin, AVB sepharose (GE), we found that, under the same conditions, different serotypes have different affinities to the resin, with AAV3B binding the best and AAV9 the poorest.
First, we used affinity purification with immobilized recombinant phage to isolate the cognate antibodies from the polyclonal rhesus monkey serum.
We used affinity purified DNMT3B antibodies to pull down DNA from formaldehyde cross-linked chromatin prepared from RKO cells.
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Another commonly used affinity-based immobilization technique for capture antibodies in immunoassay systems involves a bacterial antibody-binding protein, the two most common of which are Protein A and Protein G.
For immunostaining, we used affinity-purified polyclonal anti-GbE antibodies (Blank, Kiger, et al. 2011).
To study the distribution of endogenous LPL in rat liver we used affinity-purified chicken antibodies raised against bovine LPL.
Immunohistochemistry and double immunofluorescence labelling for IL-17 and myeloperoxidase with a widely used affinity-purified polyclonal IL-17 antibody 10, 11, 13 revealed that epidermal neutrophils were the numerically predominant cell type containing IL-17 in active plaques.
Others have used affinity-based probes that capture protein subclasses, such as kinases or serine hydrolases, 19) but these approaches also do not enable selective targeting of individual proteins.
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