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A joint pilot project between two Centers used a zebrafish microarray to identify a subset of genes that were differentially expressed in the vertebrate central nervous system in response to low level (asymptomatic) DA exposure.
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In that study, zebrafish larval cells were specifically sorted and analyzed using a zebrafish Affymetrix microarray platform.
Microarray analysis was performed using a Zebrafish (V3) Gene Expression Microarray containing 43663 probes (Agilent Technologies, The Netherlands).
However, in vivo/in vitro relationships have not yet been reported using a zebrafish model.
Microarray analysis was performed using a zebrafish-specific Affymetrix chip (15617 zebrafish probes).
Handley-Goldstone et al. [24] used a custom microarray on whole zebrafish that were 3 days post-fertilization, which had been dosed earlier in development.
Recently, Roux and Robinson-Rechavi (2008) have used zebrafish microarray and mouse expressed sequence tag (EST) data spanning, respectively, 14 and 26 developmental stages to investigate whether the timing of expression during development constrains genes' "evolvability".
This study aims to identify hepatic genes involved in vitellogenesis and regulated by E2, by using zebrafish microarray gene expression profiling, and to provide information on functional distinctive genes expressed in the liver of a vitellogenic female, using zebrafish as a model fish.
We used a custom multispecies microarray to study gene expression in wild hornyhead turbot (Pleuronichthys verticalis) collected from polluted and clean coastal waters and in laboratory male zebrafish (Danio rerio) after exposure to estradiol and 4-nonylphenol.
To identify specific gene pools affected by the knockdown of ERK1 or ERK2, and to identify possible downstream targets, microarray based transcriptome analysis was performed using Agilent zebrafish microarrays.
Wen et al. conducted a whole body male-female comparison of the zebrafish transcriptome using a cDNA microarray representing 8793 unique EST clusters [ 26].
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