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To determine if host detection of Mtb involves inflammasome activation, we used a used a panel of human monocytic THP-1 celinesnes with reduced expression of inflammasome genes due to shRNA targeting sequences (Table S1, Figure S1).
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Do not use a used toothbrush.
If you're using a used press, check that the press has clean papers and cardboard inside.
Total RNA was reverse transcribed into first-strand cDNA using a using a cDNA synthesis kit (GE Healthcare, Stockholm, Sweden).
The feasibility of the approach is illustrated using a use-case featuring a distributed file system.
I used to use a Yashica Mat and I sometimes use a Rolleiflex too.
We used to use a wide variety of technologies, now we use a handful.
First of all, getting the distillation equipment is a pain in the ass, "We used to use a copper serpentine.
I used to use a lot of cream cheese.
"I mean, it took me ages to get used to using a computer".
South Korea used to use a single government-issued modern-history textbook to teach teenagers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com