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Therefore, we have used a tissue slice culture system which allows the examination of short term effects of chemotherapy on CAFs within their natural environment of the tumor tissue [ 20].
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Slice thickness was set at 250 microns using a tissue slice thickness gauge (Alabama Research and Development) and slices were cut using a reciprocating blade at 30 rpm.
Three hundred µm coronal sections were cut using a tissue slicer from Stoelting (Wood Dale, IL, USA).
Transverse hippocampal slices (380 μm) were cut using a tissue slicer and maintained in a humidified interface holding chamber at room temperature until use.
The hippocampi were dissected free and 400 µm-thick (transverse section in hippocampus) slices were cut using a tissue chopper.
Brains obtained from E39-E40 embryos were cut under sterile conditions into 400 µm thick coronal slices using a tissue chopper (Stoelting, Wood Dale, IL).
These pieces were further cut into PCLS of 200 300 μm thickness using a Krumdieck tissue slicer filled with ice-cold KHB using the medium arm speed and blade speed.
In the present study, we isolated the forebrain from early postnatal mice and sectioned them sagittally into 350-μm-thick slices by using a tissue chopper.
The brains were then mounted on a stage using Krazy Glue, and coronal brain slices (300 µm thickness) were cut using a cooled tissue slicer (Vibratome, 2000).
Using a vibrating tissue slicer, thalamic slices (275 300-μm thick) were cut on a horizontal plane to access the thalamic reticular nucleus (TRN) and ventral basal (VB) nucleus and on the coronal plane for the dorsal lateral geniculate nucleus (dLGN); astrocyte samples were prepared from slices taken from both planes.
Next, 5 mm cylindrical liver cores were prepared with a surgical biopsy punch and sectioned to 200 μm slices using a Krumedieck tissue slicer (Alabama Research and Development, Munford, AL, USA) filled with carbonated KHB.
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