Using a tissue model, we compared temperature-time curves for four cryotherapy methods: uninterrupted freezing with nitrous oxide (N2O) and carbon dioxide (CO2), and two methods using a standard and extended version of the cough technique with CO2.
To determine the consequences of PPARγ- and PPARγ2-suppression in mouse epithelial cells in vivo with physiologically relevant epithelial-stromal interactions, we used a tissue recombination model.
Bovine ovarian tissue was used as a tissue model.
Fish tissue samples (gills, kidney, liver) were embedded in pure paraffin (Carl Roth, Germany) using a tissue processor (Model TP 1020; Leica Biosystems; Germany).
Using a tissue cage model of inflammation, the pharmacokinetic properties of marbofloxacin were established for serum, inflamed tissue cage fluid (exudate) and non-inflamed tissue cage fluid (transudate).
Total RNA was isolated using mirVana® miRNA isolation kit (Ambion, Applied Biosystems CA USA) according to the manufacturer's recommendation Tissue homogenization was carried out using a tissue tearor (Model 985370, Dremel, WI, USA).
After removal of the acetone, 0.6 ml TRI reagent (Ambion) was added to the antennae and the sample was homogenized using a Tissue-tearor (model 98370 365, Bartlesville, OK, USA).
The thenar tissue oxygen saturation (StO2) and the tissue hemoglobin index (THI), an indicator of the blood volume in the region of the microvasculature sensed by the NIRS probe [29], were measured using a tissue spectrometer (InSpectra™ Model 650; Hutchinson Technology Inc., Hutchinson, MN, USA).
The thenar tissue oxygen saturation (StO2) and the tissue hemoglobin index (THI), an indicator of the blood volume in the region of the microvasculature sensed by the NIRS probe [ 29], were measured using a tissue spectrometer (InSpectra™ Model 650; Hutchinson Technology Inc., Hutchinson, MN, USA).
The binding was also quantified using a reference tissue model with white matter as reference.
