Sentence examples for used a previously established from inspiring English sources

To compare the membrane fusion activity of full-length ATLs, we used a previously established in vitro lipid-mixing assay.

To effectively aggregate α and β cells and hydrogel beads, we used a previously established rapid aggregation method.

In this study, we used a previously established, long-term livestock grazing experiment in California oak [Quercus L.] savannas to investigate how the removal of grazing affected plant communities and soil characteristics underneath and outside of isolated tree canopies.

For these experiments, we used a previously established assay to quantify presynaptic retraction events in NMJ.

To test covalent complex formation in living cells, we used a previously established trapping assay [44].

We used a previously established echocardiography protocol [35] to determine systolic and diastolic function in the rats.

These sediment samples were evaluated using a previously established sequential extractions procedure.

DNA extraction was carried out using a previously established protocol [6] DNA was extracted from the portion of the specimen not used for microbiological culture as follows.

The esterase activity was calculated using a previously established standard curve with a fluorescein concentration range of 1 to 32 μmol/L (Fluka, Buchs, Switzerland).

Extrapulmonary lung injury was induced using a previously established double-hit model of ischemia reperfusion and fecal peritonitis [11, 12, 15].

We analyze the time evolution of non-uniform spacing distributions using a previously established semi-empirical "spacing-diffusion" law [Akamatsu et al., Metal. Mater. Trans. A 35 (2004 18155].