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Three groups were used: a normal control group that received no injections; a cryoinjured group; and a MDSC injection group of cryoinjured rats.
We investigated a total of 51 loci and found a number of alterations with respect to genomic DNA from primary cultures of human astrocytes, used a normal control reference.
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Hepatic tissue was used as a normal control for CK19 immunostaining; biliary ducts and hepatocytes were used as positive and negative controls, respectively.
One DNA that was known to have an amplified EGFR gene was used as a positive control (EGFR/ACTB=12.67±2.31) and human placental DNA was used as a normal control (EGFR/ACTB=0.55±0.06).
It is to be noted that BT-474 and MDA-MB-231 cells are HER2-positive and -negative, respectively [ 31].To confirm the effect of SUV420H2 on cell invasion, we used MCF10A, an immortalized but nontumorigenic mammary epithelial cell line widely used as a normal control for breast cancer cells.
Treatment with DMSO was used as a vehicle control to ensure the absence of solvent-induced cytotoxicity, while HMEC cells were used as a normal control to assess the effects of ACA on non-cancerous cells.
To determine genes involved in the P53 signalling pathway that were altered in metastatic melanoma, whole genome bead arrays were used to analyse gene expression patterns in 82 metastatic melanomas compared to 8 diploid melanocyte strains, which were used as a normal control.
Four rabbits were used as a normal control group.
Human Genomic DNA (Takara) was used as a normal control.
HNOKs were used as a normal control [ 22, 23].
Another group of 8 normal healthy rats was used as a normal control.
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