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Microvesicle-free plasma (pellet supernatant) was used a negative control, and urokinase (Euromedica, Diegem, Belgium) was used with a range from 0.005 U to 0.5 U. Absorbance variations were measured for 18 h at 405 and 490 nm at 37 °C with a thermostated spectrophotometer (Versamax).
Myoglobulin was used a negative control for GR chromatin occupancy: CCTCACATGGGCAGCTATTT (myoglobin forward); GCTTGTGCAAGTCCAGACAG (myoglobulin reverse).
Fluorescence-positive erythrocytes were not observed among erythrocytes used a negative control (Fig. 4a).
DNA from leukaemia Raji cells was used a negative control.
There was no increase in dye release overtime in the presence of ethanol which was used a negative control.
We used a negative control inhibitor based on Caenorhabditis elegans miRNAs not found in humans (miRIDIAN miRNA hairpin inhibitor negative control).
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No signal was observed using a negative control antibody (normal mouse IgG).
In general, all studies reported using a negative control.
GST was used as a negative control.
PBS was used as a negative control.
DMSO was used as a negative control.
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CEO of Professional Science Editing for Scientists @ prosciediting.com