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For ISH, we used a modified protocol based on Strahle et al. [86].
To expose rats to stress, we used a modified protocol of an established restraint stress paradigm [12], [13].
We used a modified protocol enriched with the CD133 antibody.
We used a modified protocol of the PCR-Based Subtractive cDNA Cloning [ 62].
To study DCs in the spleen ex vivo, we used a modified protocol from Vremec and colleagues [ 16].
We used a modified protocol of this method to study the antimicrobial activities of Mul-1867 and CHG against preformed biofilms.
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Bulk ATAC-seq libraries were generated using a modified protocol based on previous study18.
The cell lysate is then assayed using a modified protocol for the LANCE cAMP detection kit.
Images were pre-processed and analyzed using a modified protocol for DTI in SPM2.
Cerebral organoids were generated from hESCs (H9 and HUES8) and from human iPSCs using a modified protocol of Lancaster et al.40, described in the Supplementary Methods and in Supplementary Fig. 1.
To generate LSEC proteome, we isolated LSECs from normal and arsenic exposed mice respectively using a modified protocol including two-step collagenase perfusion, centrifugation and magnetic beads sorting, as described previously14,15,16.
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