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For heartbeat subtraction, analysis, and data display, we used a custom library of routines for optical imaging written in Java (Sun Microsystems, USA) and Mathematica (Wolfram Research, USA), with supplements in Matlab (Mathworks, USA).
We used a custom library of TE consensus sequences as identified above from each taxon to mask the respective filtered data set with RepeatMasker.
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RepeatMasker masked the repeat elements in the newly assembled genome using a custom library.
To identify transposable elements at the DNA level, Repeat Masker was applied using a custom library based on Repbase.
DOI: http://dx.doi.org/10.7554/eLife.01812.010 > To find out whether we could inhibit SAS-6 oligomerization, we conducted a small-scale fragment screen using a custom library of halogenated fragments (HEFLib) (Wilcken et al., 2012).
To delineate various repetitive elements present in the C. floridanus genome, RepeatMasker v 4.0 [ 102] was applied using a custom library comprising a combination of Repbase library [ 32] and the de novo repeat library customised for the C. floridanus genome constructed with RepeatModeler [ 103] with default parameters.
We used a custom GeneChip library file (CDF) based on Refseq target definitions (Hs133P REFSEQ Version 8) to provide accurate interpretation of GeneChip data (Dai et al, 2005).
We used a custom 'library' of 1,907 human transcription factor (TF) binding motifs to identify TF motifs enriched in a 'positive' set of sequences (those with DNA methylation changes), compared to a 'negative' set (a matched set of sequences without DNA methylation changes).
Consensus sequences for each of the reconstructed piggyBac-like families were used to create a custom library for a local installation of RepeatMasker.
Additionally, a pilot screen using a commercial whole genome shRNA library identified targets in the ubiquitylation pathway (data not shown), and we therefore performed a parallel experiment using a custom designed library of shRNAs directed at ∼1,000 target genes encoding mouse proteins with a function in ubiquitylation/sumolyation.
First, to construct a custom silkworm repeat database for use as a custom library file of the RepeatMasker program [ 35], we extracted silkworm repeat-related sequences enrolled in NCBI-GenBank (Release 152.0) [ 36] with a custom Perl script.
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