We use regular growth media with serum (specific to the cells being used) as the flow buffer to avoid inducing flux, which can occur when using typical PBS-BSA flow buffers, but it should not matter if the cells are kept on ice.
This study focuses on the three most closely related species to D. melanogaster (with available genomic data) to avoid using inaccurate multiple sequence alignments, which can occur when dealing with more distantly related species.
In our studies we generated biotinylated F ab' 2 anti-FcγRIIa and FcγRIIIb molecules and used streptavidin for cross-linking; the potential for non-specific ligation of receptors, which can occur when using a secondary IgG, was therefore eliminated.
Mean MPR in this study was >1.0, which can occur if patients use more than the prescribed amount of drops per dose, use more than the prescribed amount of doses per day, stockpile medication or lose a bottle or need to change their drug regimen.
The Likert scale responses were used to guard against information loss which can occur when experiencing response dichotomisation [39].
It is shown that for the same computational requirements, the derivation of the PMM is more coherent and resolves the problem of the negative variance which can occur when using other PEMs derived from the Taylor Series Expansion.
The sutures were subsequently placed as an open repair eliminating the confounding variable of poor suture placement, which can occur in using a percutaneous placement (Demetracopoulos et al., 2014; Heitman et al., 2011).
It has a hollow chamber of approximately 50 mL to prevent aspiration pneumonia caused by regurgitation, which can occur when using an SAD.
For example, wheelchair users should angle their wheelchairs appropriately relative to the target surface (20 45 degrees) to reduce the large internal rotation shoulder moments on the leading side which can occur when using a proper leading handgrip.
Directly comparing unisensory and multisensory conditions avoids the issue of subtracting activity common to both auditory and visual unimodal responses, which can occur when using the more traditional model of comparing multisensory activity to the sum of the unisensory responses (Stekelenburg & Vroomen, 2007; Teder-Salejarvi et al., 2002).
