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We will use this vector to get the solution to the problem.
We use this vector to build rotation matrix and then apply it into point cloud data in order to rotate point cloud.
We replaced green fluorescent protein sequence by mCherry sequence to be able to use this vector in DLD Tet-off POX cells, which already express green fluorescent protein under the conditions of doxycycline removal to report efficient POX gene expression.
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Transient expression of transgenic vector in HEK293T cells showed that most of the fusion protein could be efficiently cleaved, which encouraged us to produce transgenic pigs using this vector.
BHMT expressed using this vector could be rapidly purified using metal chelate chromatography.
Using this vector system, we have successfully expressed enhanced green fluorescent protein (EGFP) and firefly luciferase (Luc) in G. frondosa.
Hence, when the rake receiver is used, this vector is expressed as w Rake = e H H (3.3).
This study continues the investigation of intracellular barriers to transfection using this vector in "normal" and cancer cell lines to understand the underlying mechanisms of the selectivity.
Using this vector we achieved expression levels of ~ 450 mg/kg leaf tissue of green fluorescent protein (GFP) when the vector was delivered into Nicotiana benthamiana plants by agroinfiltration.
Using this vector, the uninduced state can be kept at background levels and induction factors of 100-fold are repeatedly obtained over months both in tissue culture and in vivo.
To achieve safe and effective in vivo siRNA delivery using this vector, the polyplex must have sufficient colloidal stability if administered intravenously or nebulized for delivery by the pulmonary route.
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