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This is the first article to use the Template for Intervention Description and Replication (TIDieR) checklist to describe a childhood obesity treatment intervention, providing valuable information to assist policymakers and practitioners wishing to implement interventions in practice.
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The intervention will be reported using the Template for Intervention Description and Replication (TIDieR).
We developed a data extraction form using the Template for Intervention Description and Replication (TIDieR checklist).
This review will therefore also code intervention descriptions using the 'Template for Intervention Description and Replication' (TIDieR 28).
cDNA preparations were used as the template for amplification using the Power SYBR-Green PCR master mix (Applied Biosystems, USA).
Rhesus genomic DNA obtained from the reference animal was used as the template for amplification using standard PCR procedures [ 20].
The T7-sgRNA PCR product was purified and used as the template for IVT using MEGAshortscript T7 kit (Life Technologies).
These fragments were used as the template for further PCR using primer pair OL-285/OL-286.
The cDNA was used as the template for PCR reactions performed using Platinum Blue PCR SuperMix (Invitrogen, Grand Island, NY).
The genomic DNA was subsequently used as the template for the fungal DNA quantification using qPCR.
The obtained pCT-hCXCL1WT-Aga2 vector was used as the template for the site-directed mutagenesis.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com