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Although both processes use the same reagents, remarkable differences related to the size, distribution, or maximal wavelength position of the LSPR band have been observed.
We also used 5 different strips for evaluating commercial antibodies and patient sera by using the method described above in Material & Methods (Western Blot Analysis) to use the same reagents and protocols to evaluate both commercial antibodies and sera.
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In the neutral tailings, using the same reagents, less than 1% of the metals were extractable.
After optimization, five laboratories tested assay performance using the same reagents and PBMC samples; similar results were obtained despite the use of flow cytometers with different configurations.
We show that ZFN-10 can function as an accurate and reliable ZFN using the same reagents and protocols used for naturally occurring and commercially available recombinant restriction enzymes.
Using the same reagents and disposables, eMAG™ adds further automation, allowing simultaneous extraction of 48 samples directly from primary tubes, and distribution of nucleic acid extracts on PCR strips or in tubes at the end of the extraction process.
Within each experiment samples were processed in parallel using the same reagents.
The RNA was then reverse transcribed using SuperScript III using the same reagents as were used for the samples assessed by microarray.
Because all RNA samples were prepared at the same time using the same reagents and methods, this shows that the aptamers promoted neurite outgrowth based on their particular sequences and structures alone.
For comparisons, samples of different genotype were always examined in parallel, using the same reagents and cytometer settings, to eliminate differences resulting from day-to-day variations in instrument performance, reagent stability, etc.
When comparing PDF Immuno-reactivity levels between genotypes, care was taken to insure that the immunocytochemistry was conducted in parallel using the same reagents, aliquots of antisera, and exposure and rinse times.
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