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Although not all studies use the same PCR protocol, the concordance between HC2 and PCR testing across various studies is generally high, usually exceeding 80% [ 17- 20].
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The intron amplified using the same PCR recipe as above and PCR program- ii.
RT reactions that were conducted without primers (-p) also generated measurable amounts of PCR products using the same PCR primers used to detect sense and antisense MYH7 RNA (Fig 1).
mtDNA with TTGE banding pattern abnormalities then underwent direct DNA sequencing using the same PCR fragment; detected mutations underwent repeat PCR and sequencing for confirmation.
The amplification reaction was performed using the same PCR profile as mentioned above and the PCR amplicon was cleaned as mentioned above.
After the extraction of organelle DNA, PCR products were re-amplified with the conservative primer pairs by using the same PCR programs as mentioned above.
The PCR products were purified using the Wizard SV gel and PCR cleanup system (Promega) and then sequenced using the same PCR primers.
All hANT knock-in DNA fragments were constructed using the same PCR strategy described above.
Using the same PCR conditions plasmids containing RBSP3 and RASSF1A were amplified from E.coli and no mutations were discovered arguing against generation of mutations during PCR amplification.
SNP analysis of these 6 extractions were compared to previously analysed European SNP data using the same PCR assays and were found to be the same.
All reaction templates were normalized using the same PCR conditions with GAPDH expression using primers: forward 5'-accacagtccatgccatcac-3' and reverse 5'-tccaccaccctgttgctgta-3'.
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