NOCA-1 antibodies were affinity purified using the same antigen after cleavage of the GST tag as previously described (Desai et al., 2003).
Two booster immunizations using the same antigen emulsified in incomplete Freund's adjuvant were carried out on days 14 and 28.
Rabbit antibody against SCML2 was generated using an antigen consisting of a central region of SCML2 fused to GST and affinity-purified using the same antigen fused to a 6xHis tag.
Using the same antigen, we previously found that the specificity of the SeroCP IgG antibodies was 38.5% and that this test was not sufficiently specific to distinguish between IgG antibodies to Cpn and to Ct [ 29].
Results obtained with the ELISA based on recombinant swine HEV genotype 3 rΔ111ORF2 ORF2 capsid protein (ELISA-ORF2) were compared with a Western blotting (WB) test using the same antigen and with a commercial ELISA kit.
In contrast, vaccines using the same antigen with aluminum hydroxide and MPL (AS04) or in an oil-in-water emulsion (AS03) induced high levels of antibody but failed to protect against infection.
In a similar procedure for lymphocyte stimulation, using the same antigen preparation (peroxide-treated tumour cells) and tumour target (M74 cell line), we repeatedly observed defective stimulation when DCs were from patients with breast cancer.
The anti-CCP antibody analyses used the same antigen as used in our earlier investigation of anti-CCP2 antibodies of the IgG isotype, and the IgG anti-CCP antibody analyses is consequently a reiteration of our earlier published anti-CCP2 data [ 1].
Similar results were found when F2 hybrid mice of susceptible BALB/c and resistant strains were immunized and tested for arthritis- or spondyloarthropathy-associated QTLs using the same antigen, immunization protocol, and scoring system (visual and histology) and when MHC- and age-matched animals were housed in the same room, occasionally for more than half a year [ 9, 11, 23, 24, 29, 66].
All VLP antigen concentrations were standardized using the same antigen-capture described above in antigen characterization section.
