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Also, because we use the same antibody (separately labeled by donor or acceptor fluorophores) against DR1, we neither induce large-scale antibody mediated aggregation of DR1 nor generate false FRET signals, since each DR1 molecule can be bound only by one antibody molecule.
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For future studies, contributing diagnostic centres will use the same antibodies, protocol and automated staining platform.
Since EpCAM is not equally expressed in all tumor cells [ 15, 19], ideally, it would make better sense to use the same antibodies to enrich and identify CTCs.
IHC results for HER2/neu vary with replicate testing using the same antibody clone and when alternate clones are utilized.
Furthermore, they used the same antibody as Boström et al.1, designated pAb-E here, which was raised against a C-terminal sequence that is not included in irisin.
The device uses the same antibody technology that medical labs use in testing for things like flu viruses.
Each procedure was repeated a minimum of three times to validate the results and minimize any experimental errors using the same antibody stock.
In line with others using the same antibody [13], we found that circulating CD4+ T cells were effectively deleted.
All combinations of Myc-, V5-, or un-tagged aSyn molecules were stained using the same antibody combination as described above.
Other studies performed in mice have also reported CSPG deposition post trauma using the same antibody [57].
Since we used the same antibody that was used in the Cao et al. [12] study, these differences may be due to species differences or differences in fixation.
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