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In order to use the reference set, the events need to be translated into codes.
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Using the reference set G T 1, the performance achieved with ComplOverlap was similar to that observed using the graphic oracle, which used the three extractors.
I used the reference set in the Ehum.cut file that is distributed with EMBOSS [33].
We annotated all the modules using the reference set named PlantGSEA (23).
Similar results were obtained when we used the reference set from MitoP2 [ 57] as a source of mitochondrial proteins.
We also plot receiver operator characteristic (ROC) and precision-recall curves using the reference set to determine true and false instances.
We labeled drug event associations as negative if there was lack of evidence, which in itself is something that may rapidly change over time; checking of the absence of evidence should always be carried out prior to using the reference set.
Therefore, we first evaluated the distribution of multispecies conservation scores (PhastCons score) [41] and regulatory potentials (RegPotential score) [42] for positions in the functional and non-functional STAT3 TFBSs detected by STAT-Scanner using the reference set of 22 genes (Table S1).
As the choice of threshold for these bootstrap values has a significant influence on the outcomes of subsequent analysis, we compared two previously implemented confidence value thresholds of 50% [30] and 80% [40] using the reference set of 7,208 near full-length 16S rRNA genes from human fecal microbiota sequenced by Dethlefsen and colleagues [14].
CAI values were calculated using the reference set of optimal codons from S. cerevisiae.
In each case the number of true positives (TP) and true negatives (TN) were calculated using the reference set of human genes included in Figure 1.
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