Using the RCA-TAR method, we constructed a set of different alphoid DNA arrays that have been used to elucidate the structural requirements for de novo kinetochore formation in human cells [ 24- 27].
More than 104 millions of paired-end reads from the sample 9, 161 millions from the sample 10, and 120 millions from the sample 13 were obtained using the RCA-seq.
Additionally, 114 full DNA-A and 41 full DNA-B sequences (see Additional file 1: Table S1 for details) were obtained from total plant DNA extracts using the RCA-RFLP strategy [ 42].
Prior to the growth of AlN films, the Si substrates were cleaned using the Radio Corporation of America (RCA) cleaning process and then dipped into a 2% hydrofluoric acid (HF) aqueous solution for 2 min to remove the native oxides.
Leaf tissue (10 g) was collected for plastid isolation based on the sucrose step gradient centrifugation method of Palmer [ 83]. Isolation was followed by whole plastid genome Rolling Circle Amplification (RCA) using the Repli-g RCA kit (Qiagen, Inc).
After that, 10 g of young leaf tissue was collected for cpDNA isolation based on the sucrose step gradient centrifugation method by Sandbrink et al [ 62]. Isolation was followed by whole chloroplast genome Rolling Circle Amplification (RCA), using the Repli-g RCA kit (Qiagen, Inc).
Therefore, a variety of biomolecular targets such as genes, microRNAs, proteins, with ultra-low abundance have been successfully detected using the developed RCA-based electrochemical biosensors.
Plug your audio interface into your computer's USB port (or FireWire port for some interfaces), and use the appropriate cable to connect your mixer's Tape Out or Line Out jack to your audio interface's line input the setup above uses an RCA-to-stereo minijack cable.
Chloroplast DNA was subjected to rolling circle amplification (RCA) using the Repli-g kit following the manufacturers instructions (Molecular Staging Inc, New Haven, CT .. Purified RCA products were subjected to nebulization, followed by end repair and size-fractionated by agarose gel electrophoresis to obtain fragment lengths ranging from 2.0 3.5 kb.
