In a previous study [ 27] that determined the composition of the SN used in the present one, IONPRODUCT, which was developed by Peter Shellis [ 62], was used.
Specific primers (see additional file 1) were designed for all the validated candidates and the zooxanthellae-specific primers ss5Z (5'-GCAGTTATAATTTATTTGATGGTCACTGCTAC-3') and ss3Z (5'-AGCACTGCGTCAGTCCGAATAATTCACCGG) identified in a previous study [ 57], were used in the present one.
Therefore, when using the present method, one should take into account that about half of the positive cells will actually appear as false-negatives (i.e., expressing the transgene but not the marker).
For controls, we included data on healthy blood donors from our initial study, which used identical methods as the present one [ 17].
There were also several low-coverage genomes available, but their lack of many annotated features made their use in studies like the present one very inconvenient [ 64, 65].
In contrast to other cell models, the present one uses some a priori structural configurations in conjunction with the distributions of coordination numbers and polar angles in a random packing.
The difference between the outcome measures used in that study and the present one precluded the possibility of a meaningful comparison.
This study [ 17], similar to the present one, used TMA for the reading of the immunohistochemical analysis, but employed different criteria for the interpretation of HER1, HER3, and HER4 expression, in addition to also employing fluorescence in situ hybridization (FISH).
But it would take a bolder secretary of state than the present one to use them.
The statistics used for each pair wise comparison between sites were: Mutual Information MIis is a widely used statistic in several fields, including the present one[ 26].
