After determination of a normalisation factor from the set of reference genes, the raw data of the QPCR runs were imported into qBase version 1.3.5 [ 37], an Excel (Microsoft) based tool which calculates relative gene expression normalised using the normalisation factor generated with geNorm.
Absolute quantification was performed using the standard curves and the data for prestin and myosin VIIa were normalised in GENorm using the normalisation factor calculated from TBP, Ywhaz and Pla2gl2a, the three most stably expressed control genes (32).
The interview guide was developed using the normalisation process theory (NPT) (May et al, 2009).
To ensure comparability of the data sets, we corrected for different sampling depths using the normalisation method suggested in [ 9].
We used the normalisation process model (May 2006) to order the data collected in consultation meetings and to inform our strategy and actions.
This paper describes using the normalisation process model [ 13] to establish, integrate and evaluate a chiropractic service in a rural, Indigenous Australian community.
Although this approach gives the expected results (as shown in Figure 5), it is an involved method since it uses the normalisation curve in the frequency domain in order to derive the appropriate inverse filter.
Extracted data were analysed using the Normalisation Process Theory framework, detailing the challenges impacting on telestroke during four stages of system: 1) development, 2) implementation, 3) use, and 4) evaluation.
The element 'context' was explored into more detail using the Normalisation Process Model [ 24] to identify factors that affect the success or failure of delivering and implementing the intervention in a dynamic and complex primary care setting.
Interviews were transcribed verbatim and data was analysed using a two-step approach using an initial thematic analysis, and a secondary analysis using the Normalisation Process Theory concepts of coherence, cognitive participation, collective action and reflexive monitoring with respect to the implementation of CC in primary care.
