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Prior to use the mobile phase was filtered through 0.45 mm Millipore membrane filters and degassed by sonication in an ultrasonic bath.
Before use, the mobile phase was filtered through 0.45 µm (pore size) filter (Millipore, Bedford, MA, USA), and degassed under vacuum.
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The peptides were separated using the mobile phase comprising of solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) in a gradient elution mode with a total run time of 100 min.
The analysis was performed using the mobile phase: 0.1% acetic acid in acetonitrile and 0.1% acetic acid in water (90 10, v/v) under column temperature 25°C.
A standard stock solution of brimonidine tartrate (1 mg/mL) was prepared using the mobile phase.
Cordycepin and adenosine were separated by gradient elution using the mobile phase with the composition of water methanol (95.0/5.0 to 58.4/41.6 in 20 min, v/v).
Fraction Fr-3-2 were further purified by HPLC using the mobile phase of 33%CH3CN/H2OO to give compounds 8 (18 mg) and 6 (16.9 mg).
Standard calibration solutions (5 to 1,000??g?mL?1) for the assessment of linearity were prepared from this stock solution using the mobile phase.
Fr-IV was purified by silica gel column using the mobile phase of CH3Cl/CH3OH (3:1, v/v) to afford compound 17 (162 mg).
Separation method of ibuprofen, phenylephrine HCl, and cetirizine from soft gels was reported using the mobile phase of acetonitrile and hexanosulphonate (Cosmes and Rodriguez [2012]).
Twenty microlitres of the sample was injected manually in the injection port and analysed with a flow rate 1 ml/min at 60°C with UV detection at 220 nm using the mobile phase of methanol/water (45:55, v/v).
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