In any case, always use the higher range measurement protected by the higher fuse rating and be careful.
The insulin content of the incubation medium was analysed using the high-range HTRF insulin kit (62INSPEC, Cisbio Bioassays, Codolet, France) and fluorescence resonance energy transfer was measured on an Envision plate reader (PerkinElmer, Cambridge, UK).
If your home is new or well insulated, you can use the lower of the 2 numbers for your climate zone; if it is older or poorly insulated, use the higher number of the range.
The effects of PKA in the Isoprenaline group also indicate that,although the dose used was in the high range of values reported in the literature, Isoprenaline did not result in complete saturation of the PKA sites.
In this study, we applied low-pass filters not only with f c = 1 Hz but also with f c = 1.5 Hz to emphasize the dissimilarity between the RFs estimated at two stations by using the higher frequency range.
The amplification reactions were performed using the high fidelity "Long Range" Taq DNA polymerase (Qiagen) and following the manufacturer instructions: preheating at 93°C for 3 min, then 35 cycles of denaturation at 93°C for 15 s, annealing at 62°C for 30 s and extension at 68°C for 7 min. All of the PCR products were visualised on agarose gels, cloned and sequenced on both strands.
After centrifugation (16,000× g, 10 min) insulin was detected in the supernatant fraction using the Mouse High Range Insulin ELISA (Alpco, Salem, USA).
This holds true within both the low and the high range of contraceptive use.
The molecule markers used were the high-range rainbow molecular weight marker (Amersham Pharmacia Biotech) for radiolabelled samples and the Seeblue Plus 2 prestained standard (Invitrogen) for nonlabelled samples.
In order to set the normal range of B, E, R, T RQ values for scaling the corresponding mRNA expression in tumors, we used the highest populated 3-cycle range in each RQ series of normal samples (Additional 1 Table 3).
