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Protocols ending in A use the CAGE data as reference, while the ones ending in B use the gene set.
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Gene set analysis was performed using the Gene set enrichment analysis (GSEA) software V2.0.2 (http://www.broad.mit.edu/gsea) (Subramanian et al, 2005).
Enrichment scores for each and every compound in the database were computed using the gene set enrichment analysis algorithm [11].
The clusters of genes identified by CAGED were analyzed using the gene set enrichment analysis in David Bioinformatics resources Huang da et al., 2007 [33] (http://david.abcc.ncifcrf.gov/home.jspand), and Hosack et al., 2003 [34].
Genes were loaded using the gene set format with FI annotations and linker genes.
Hence, we performed the analysis using the gene set permutation option.
Enriched pathways were evaluated using the gene set enrichment analysis of GeneTrail.
First, using the gene set expression comparison tool, 302 pathways (as defined by Biocarta) were analyzed.
We first classified the core-bound genes into different gene families using the gene set enrichment analysis (GSEA) software [ 11].
We used the gene set enrichment software (GSEA) software [ 80, 81] to identify pathways associated with β-catenin siRNA treatment.
Pathway analysis was performed using the gene set comparison tool of BRB-ArrayTools Version 3.6 (Biometrics Research Branch, National Cancer Institute).
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