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It is therefore important for the model-theoretic actualist to use the "free" quantification axiom FUI of Adams' logic.
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Use the free one.
Plasma non-esterified fatty acids (NEFA) were quantified using the Free Fatty Acid Quantification Kit (Biovision Inc ,Mountain View, CA, USA).
FFA concentration was analyzed using the Free Fatty Acid Quantification Kit from Biovision, as described previously [ 7].
Quantification was carried out using the label-free quantification algorithm implemented in MaxQuant using a minimal ratio count of 2 and the "match between runs" option enabled.
Protein identifications were extracted and a measure of normalized amino acid coverage was used as label free quantification.
Data was analyzed using "Label-free quantification" checked, and the "Match between runs" interval was set to 2 min.
Although a study using label-free quantification to analyze the ubiquitylated proteome of rat brain tissue was recently published [ 41], to our knowledge, our study represents the first integrated quantitative analysis of endogenous global protein and ubiquitylation in tissue using iTRAQ labeling.
Quantification may be achieved by optical density measurements on 2-DE gels or by MS peak area determination using label-free quantification [48].
Protein profiling using label-free quantification was conducted to identify proteins whose expression was altered by HPV infection.
The AMT tag strategy [19] was used for label-free quantification of MS features observed in the LTQ-Orbitrap Velos analysis of the individual CSF samples from normal, CFS and nPTLS conditions.
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