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It is highly recommended, when looking for homologies, to use the BLAST tool, which is more sensitive rather than other alignment tools such as BLAT [ 34].
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In addition to these fragments, an EST (Genbank Acc. No. GW403182.1) was derived from the NCBI database by using the BLAST tool (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
All sequences were blasted using the BLAST tool of VectorBase (http://www.vectorbase.org/Tools/BLAST) against the An.
Primers were designed on the basis of known sequence information of Nipponbare genome (TIGRv3) by using the blast tool of Gramene (www.gramene.org).org
Using the BLAST tool, Collembase can be queried for genes valuable for phylogenetic inference.
The nucleotide sequence of defensin PaDef cDNA and the deduced amino acid sequence were analyzed using the Blast tool in the NCBI (http://blast.ncbi.nlm.nih.gov/).nih.gov/
Using the BLAST tool, their current annotation status was manually checked against the Uniprot database [ 30], and their domain architecture checked using the Pfam domain organization database.
Primers specificity was verified using the BLAST tool from the NCBI and a dissociation curve analysis was performed after the PCR reaction.
A Genbank homology search using the Blast tool showed 89% identity at the amino acid level with a C3'H isolated from Ocinum basilicum (AAL99200.1).
Sequence conservation analysis of APC and activators genes was conducted using the BLAST tool with default curve calculation parameters; amino acids sequence "conservation similarity" of 50%.
These 21 and 22 nucleotides, which were the gene tags, were analyzed as queries for aligning them to reference genes in the database using the blast tool (BLAST 2.2.23).
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