Three azo-reductase genes were amplified from genomic DNA of P. aeruginosa (isolate B1) by PCR using specific sets of primers.
Quantitative real-time-PCR was performed applying LightCycler technology (Roche) [10] using specific sets of QuantiTect Primer Assays according to the manufacturer's instructions (Qiagen, Hilden, Germany).
In conventional information systems, the majority of text information retrieval and discovery methods are based on using specific sets of pre-defined document metadata, e.g. keywords or indexes of terms characterizing the texts content.
Using specific sets of cell surface markers, these cells can also be isolated from murine and human lung and tested in 3D culture systems and in vivo transplant assays.
To date, three dominant types of cellulase systems have been identified, each using specific sets of enzymatic synergisms [ 2].
All the models showed good results with accuracy varying from 69% for Delavirdine to 89% for Lamivudine when using specific sets of rules and from 69% for Delavirdine to 88% for Lamivudine when using generalized rules.
RT-PCR was performed on total RNA isolated from the CML476 (WT) and SDP8 strains grown in YPD in the presence of doxycycline (100 μg/ml) for 8 and 24 h using specific sets of primers (Table 4 ).
We performed real-time PCR (TaqMan, Applied Biosystem, Carlsbad, CA) as described previously (Crespillo et al., 2011a; Decara et al., 2012) using specific sets of primer probes (supplementary material Tables S2, S3).
To confirm the function of mutants used in the study, genomic DNA was digested using a DNA methylation sensitive restriction enzyme and amplified using specific sets of primers (chop-PCR) targeting 5S rDNA repeats and AtSN1 to assay for the DNA methylation status at these loci.
Further, every application is site dependent and uses specific set of indicators.
