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Modern cell biologists typically use reporter genes either alone or co-expressed with a protein of interest to facilitate the localization or quantification of protein expression.
To successfully use reporter cell lines to follow and track cells it is important not only to ensure stable activity of the promoter but also to rely on strong promoter activity.
We and others often use reporter gene transcription to evaluate nitrogen-responsive regulation in yeast and other organisms.
We adapted the technique to use reporter constructs, so that changes in transcription from different promoters could be assessed at single-cell resolution.
Therefore, while multiplexing strategies are highly desirable, it can also be impractical to use reporter gene mice in a research study when the breeding schemes of combining different genetic loci into the same animal become too time consuming and costly.
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General managers have been known to use reporters to stir another team's interest.
"We'll still use reporters where that's been proven to be the best way," he said.
Expression studies often use reporters of promoter activity, such as LacZ [3].
The aphII gene is the most extensively used reporter system in Streptomyces, however, this useful system was sometimes blamed for some non-understood paradoxical behaviour.
Reports submitted by consumers were identified using reporter field.
Previous reports on the penetration depth of SERS used reporter groups attached at fixed distances from the surface.
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