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Molecular biologists use recombinant methods to engineer heterologous DNA molecule.
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The catalytic domain of the YscN protein was made using recombinant methods and its ATPase activity was characterized in vitro.
Of the 39 unique eukaryotic membrane proteins, only 17 were produced using recombinant methods, nine of these in yeast systems (Pichia pastoris and Saccharomyces cerevisiae) [ 9 17], four in insect cells [ 18,19,20] and four in E. coli [ 21 24].
In the context of immunoproteomics, such protein microarrays are complimentary to approaches using non-recombinant methods to discover and characterize bacterial antigens.
One possibility is that proteins that are difficult to express using recombinant DNA methods are less likely to be characterized, and it is possible that their homologues from other species might also be difficult to express using recombinant DNA methods.
The conventional panning method is to use recombinant antigen coated on a solid substrate; usually a microtiter plate.
The problems associated with this lability have ostensibly not been overcome through using recombinant expression methods.
Specificity of TRAIL staining was confirmed by antibody absorption, using recombinant human TRAIL, according to a method published previously [ 34].
To create in vitro nucleosome positioning maps, high molecular weight C. elegans genomic DNA was digested into fragments by either Hinc II or Rsa I restriction enzyme and reconstituted into nucleosomes using recombinant histone octamers (Methods).
There have been several attempts to develop effective LLO purification methods using recombinant Escherichia coli strains [ 5- 9].
Enzyme 2, the Sec → Cys mutant of mTR, was produced using standard recombinant methods previously reported by us.
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