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Here, we use promoter capture Hi-C to identify interacting regions of 31,253 promoters in 17 human primary hematopoietic cell types.
We demonstrated that macrophages preferentially use promoter F and that E2 treatment augments total ERα mRNA and ERα46 protein expression.
In the present study we have demonstrated that Arabidopsis calluses and cell suspensions can use promoter DNA methylation to repress specific single-copy genes that are unmethylated and expressed in differentiated cells from various origins.
An alternative approach is to use promoter expressed short hairpin (sh RNAs in gene therapy settings.
Plants require both the TATA box and the USE promoter (similar to the PSE element in vertebrates) with polymerase specificity determined by the spacing between the two elements [ 45].
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"It's a bad idea to use promoters.
"We won't use promoters or e-mail out mass invitations," said Nur Khan, who was an owner of Wax and the bar Sway, and who says he is friends with the likes of Kate Moss, Mick Jagger and Primal Scream.
However, because most tet-regulated mouse strains use promoters of genes expressed in multiple tissues, to achieve exclusive expression in an organ of interest is often impossible.
Since the CMV promoter is the most commonly used promoter in gene therapeutic approaches, primers were designed which hybridize with the human CMV promoter sequence.
For generating proFLS2::FLS2-GFP, 912 base pairs of FLS2 promoter, upstream of ATG was amplified using promoter specific primers and fused to full length CDS of FLS2 gene (Supplementary Table 3).
The model describes foreign protein expression under the control of the polyhedrin promoter, which is the most commonly used promoter in this system.
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