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The disagreement, however, can partly be explained by the use of various CGH platforms, variations in resistance classifications, heterogeneous tumor materials, and the use of various cell lines.
The use of various cell types with their corresponding PACE4-knockdown equivalent allowed the depiction of a clear correlation between the peptides retained by the cells and their levels of PACE4 expression even when comparing all cells together).
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Potential options include renal regenerative medicine through the use of various cell-based therapies as well as de-novo organ generation via bioengineered scaffolds.
Virus stocks were generated and used for transduction of various cell lines as specified in the Supplemental Experimental Procedures.
Room temperature photoluminescence has been also used to monitor the effect of various cell fabrication process steps on the local recombination activity of defects.
Total viable cell numbers harvested from each organ were determined by Trypan blue stain exclusion and were used to calculate the absolute counts of various cell populations.
Confocal laser microscopy was used for looking at the expression of various cell specific markers tagged with Green Fluorescent Protein (GFP).
As a supplement to this technique, RT-qPCR has been used to establish the expression profiles of various cell surface markers in equine MSCs [ 39, 42].
Nucleofection has been used for transfection of various cells [ 23, 29].
Much of the evidence was obtained in the in vitro studies using primary cultures of various cells from the developing brain [ 67, 156] and experiments with brain perfusion [ 53, 54].
In this study, we aimed to investigate whether the detection of CTC by a cytokeratin 20 reverse transcriptase-polymerase chain reaction (CK20 RT-PCR) may be influenced by the use of various Epithelial Cell Adhesion Molecule (EpCAM) antibodies for immunomagnetic isolation of CTC.
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