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During the Civil War the Union Army made extensive use of transposition ciphers, in which a key word indicated the order in which columns of the array were to be read and in which the elements were either plaintext words or code word replacements for plaintext.
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In general, the use of DNA transposition strategies alleviates the requirement of finding appropriate restriction sites close to an exon of interest.
The CLoNe method is largely aided by use of piggyBac transposition (Ding et al., 2005; Woltjen et al., 2009).
The general procedure was identical to Experiments 1 and 2. The use of the transposition task in Experiment 2 had not significantly led to a reduction inside biases.
Use of co-transposition analysis could provide direct evidence for MITE transposition and able to confirm cross-mobilization between MITEs and the related autonomous elements.
However, the new difficulty in the infinite dimensional setting is how to explain the stochastic integral (int _{0}^{T}Lambda (t dW t)) that appeared in (4) because for this case Λ is an operator-valued stochastic process, and therefore one has to use the theory of transposition solution for operator-valued backward stochastic evolution equations (Lü and Zhang 2014; 2015).
We made use of PiggyBac (PB) transposition [ 13] to introduce a transgene driving Nanog expression under control of a CAG promoter (PB-Nanog) in a clonal line of MEF-derived pre-iPS cells.
In principle, insertion mutant libraries can be generated by the combined use of in vitro transposition mutagenesis and simultaneous targeting of a mixture of genomic fragments, as shown with yeast and fungi [ 28, 29].
It has also been proposed that the propensity for HT could be related to the mechanism of transposition used (see Schaack, Gilbert and Feschotte 2010 for review).
Class I TEs use an RNA-mediated mode of transposition and are further divided into two subclasses, including LTR and non-LTR retrotransposons.
But his radical use of language and his transposition of the banal and the unnerving set a precedent.
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