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We demonstrate the use of this protocol on both a protein and a DNA case study.
"Use of this protocol should... be discontinued," says the University of Manitoba's Alan Jackson in a 2013 review published in the journal Antiviral Research.
We have developed a new two-substrate assay/analysis protocol that provides PON1 status without use of toxic OP substrates, allowing for use of this protocol in non-specialized laboratories.
During extensive diagnostic use of this protocol in an outbreak of CSF in wild boar in Germany, some C-strain positive field samples were found to give negative results in the C-strain "Riems" specific real-time RT-PCR, but positive results in a pan-CSFV real-time RT-PCR system.
So, even with the most recent patches in place, the continued use of this protocol is a security concern.
Thereby, the patents expire in 2017, allowing the use of this protocol to obtain recombinant glucagon.
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Use of this alternative protocol does not affect the sensitivity or specificity of the method.
Use of this testing protocol ensures timely identification of all STEC infections (2, 5 ).
Positive benefits have been achieved through use of this treatment protocol.
We therefore did not assess the interobserver variability in the use of this multifactorial protocol.
The Consortium aims to assist other international research groups in the use of this proposed protocol to allow further cross-cultural comparisons and increase statistical power by enabling meta-analyses using individual patient data.
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