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Here, we demonstrate a novel use of TALEs in combination with single-cell gene expression profiling to investigate the consequences of transcriptional perturbation of developmental regulatory networks.
Here, we present the use of TALEs (fused to transcriptional effector domains) designed to target conserved regions within haematopoietic TF cis-regulatory elements as an efficient tool to regulate target gene expression.
In summary, we have validated the use of TALEs targeting conserved cis-regulatory elements as an efficient, multifaceted tool to modulate endogenous gene expression and study TF regulatory network perturbations in single cells, and in doing so we have uncovered a role for PU.1 in haematopoietic specification.
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The simplified TALE assembly combined with multiple choices of vectors will facilitate the broad use of TALE technology.
Wollstonecraft's extensive use of inset tales encourages her readers to construct a moral narrative out of their own lives, with a predetermined ending.
— what's most interesting apart from Kadare's use of folk tales and dreams is its gender politics.
This painting reveals three new emergent themes in his practice, said Ms. Ho — the use of fairy tales and fiction to describe national narratives, the placement of Thai symbols in a monochrome palette, and the use of toy objects that resonate with a Thai audience.
In his use of fairy-tale characters in the show, he said the company encouraged his creative team to make the likes of Snow White and her dwarfs their own.
The scale of alleged theft implied by Yatseniuk in a speech to parliament was jaw-dropping, even for a population now used to tales of Yanukovich's extravagance and lavish lifestyle, including his luxury residence outside Kiev.
We are so used to tales of diplomacy ignored or even scorned that it comes almost as a shock that a skilled, persistent and patient diplomat can achieve great things for our country and the world.
The use of the word "tales" is particularly diminishing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com