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Simple semi-analytical mathematical solutions were obtained, which avoid the use of surface coverage in computations.
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Our previous work described in details the properties of adsorbed lysozyme layers as function of surface coverage, using a combination of QCM-D and DPI [16].
The important thermodynamic properties viz., limiting heat of adsorption at zero coverage, entropy, Gibbs free energy and isosteric heat of adsorption as a function of surface coverage were evaluated using van't Hoff and Clausius Clapeyron equations.
The degree of surface coverage of adsorbent covered by lead ions was calculated using theta =1 - frac{C_{mathrm{e}}}{C_o} (4).
In order to obtain the isotherm, the degree of surface coverage θ was calculated from the weight loss measurements using Eq. 1.
The degree of surface coverage, for the different systems studied, was computed from the weight loss measurements using Eq. (1) and presented in Table 1.
Degree of surface coverage.
The degree of surface coverage by nanoparticles mainly depends on the effectiveness of APTES microcontact printing and on the concentration of nanocages used for immobilization.
However, a comparison of surface coverage alone is not sufficient in order to understand the diverse layer properties produced with the same surface coverage.
For determination of surface coverages, 5′-fluorescein, 3′- CH2 3-thiol-labeled oligonucleotides were used for preparing the DNA–nanoparticle conjugates [ 21].
Limiting heat of adsorption at zero and isosteric heat as a function of the surface coverage were evaluated using Clausius Clapeyron equations.
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