Exact(2)
However, the study of expression of genes in strawberry requires the use of suitable reference genes.
However, accurate and reliable interpretation of RT-qPCR results depends heavily on the use of suitable reference genes for normalisation to eliminate or minimise non-biological variation between test samples.
Similar(58)
A few studies have been performed using sequence-based approach for the identification of suitable reference genes.
The 01APPRD tobacco sample was used as an independent quality control sample due to the unavailability of suitable reference material with a comparable matrix.
This sample was also used as an independent quality control sample due to the unavailability of suitable reference material with a comparable matrix.
Because selection of suitable reference genes is important for qPCR assay, four commonly used reference genes, actin, EF-1α, EF-TU, and PP2A, were evaluated with geNorm [ 46], DeltaCT [ 47], BestKeeper [ 48], Normfinder [ 49], and RefFinder [ 50].
However, currently their use is limited by conflicting data between studies due to lack of standardisation in methodology and the lack of suitable reference genes for normalisation.
The stability of housekeeping gene expression was tested in both species using geNorm and the NormFinder visual basic applications, two of the most diffused algorithms for the statistical determination of suitable reference genes in a qRT-PCR experiment.
The paucity of suitable reference genomes is a major obstacle in the interpretation of metagenomic data.
The selection of suitable reference alleles is a crucial step in the optimization of RSCA.
A systematic exploration of suitable reference genes in Brachypodium is presented here.
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