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This relative quantification was reliant on the use of several reference genes: 18S RNA, G6pdx, Gapdh, Tcea, Tbp.
Good practice in data normalization for gene expression analysis therefore relies on the identification, experimental validation and use of several reference genes.
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A selection of several reference genes used simultaneously can also be a good way to further increase reliability of the resulting data [ 50, 51].
We can also use the multiplex advantage of averaging I0/ I4 ratios of several reference peptides both to help define reference peptide anomalies and to improve precision.
When I translated Ludwig's Harig's German-language novel The Trip to Bordeaux, which is chockablock with unattributed quotes from Montaigne's essays, I made simultaneous use of several brilliant reference books available at the wonderful public library in Berlin, Germany: the Staatsbibliothek preußischer Kulturbesitz.
Use of several different reference sets is important because not all markers are equivalently easy to phase.
Compared with the MC-RAMP method which took use of several kinds of reference signals proposed by Husøy et al. [ 33] and Eilevstjønn et al. [ 20], the residual component of artifact could be further suppressed and the reliability for detecting a nonshockable rhythm was markedly improved.
After excluding references without an abstract and redundancies arising from the use of several databases, 208 potentially relevant references were identified.
In the analysis of RNA-seq data it is customary to use one of several available reference genome annotations when mapping sequence reads and calculating FPKM.
This translation takes place at different time scales and to different degrees of accuracy but leads to the parallel existence and use of several egocentric and allocentric frames of reference [5, 6].
Since the reference set used is an important determinant of the quality of the CN data, we purposely used several reference sets for each sample to obtain a wide range of quality in the CN data.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com