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Our use of sequence tags is distinguished from other methods by the following points.
Because of the shortcomings of shotgun-methods in soil metagenomes, we have championed the use of sequence tags to bypass these technical problems.
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The other used a small number of sequence tags on one of the two amplification primers to examine microbial diversity using paired-end Illumina sequencing [12].
We identified regions of local enrichment of sequence tags using a hotspot detection algorithm essentially as previously described [ 55, 77] with a false discovery rate (FDR) of 0.1%.
In contrast, using GeF-seq, the distributions of sequence tags on the plus and − strands overlapped in the middle of the two ChAP-seq peaks (Fig. 3B).
The starting point of our procedure was the use of expressed sequence tags (ESTs) instead of EST contigs that could lead to irrelevant annotations resulting from assembling errors.
Up to date many groups have presented genomic analyses of alternative splicing by use of expressed sequence tags (EST, [e.g. [ 21- 23]] or microarrays [ 20]. Most of these results are now available in databases [ 17].
Coupled with the use of expressed sequence tags, PCR led to the discovery of many more genes than could be found through traditional biochemical or genetic methods and opened the possibility of sequencing entire genomes.
Dr Venter pioneered the use of expressed sequence tags as a new way to identify human genes, and went on to lead the private initiative to sequence the human genome, completed in 2000.
One of the most common strategies adopted for SNP development in non-model organisms is the use of Expressed Sequence Tags (ESTs) as a resource for SNP marker detection [ 11- 14].
The use of expressed sequence tag (EST) and RNA-sequencing data from more tissues or stages would supply the gaps of the RT-PCR assay.
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