Sentence examples for use of sequence coverage from inspiring English sources

While distinct in their approaches, they are most comparable in design (making use of sequence coverage and soft clipping).

However, chorion genes are known to undergo somatic amplifications in ovarian follicle cells (Claycomb and Orr-Weaver 2005), and the use of sequence coverage to correct for "missing" copies can be misleading in these cases.

Individual AGC files contain source RNA sequences and predicted amino acid sequences, as well as supplementary fields that provide the best human RefSeq BLAST hit used for analysis of sequence coverage, percent sequence coverage of the best BLAST hit, and percent sequence identity between human and Ambystoma amino acid sequences.

For each read, positions of the strongest BLASTN hit on the BAC were used for graphical representation of sequence coverage.

The method does not rely on a high quality reference genome, high depth of sequence coverage, use of inbred strains or parentage assignment of SNPs.

The latter was used to increase the depth of sequence coverage to ensure reliable detection of polymorphisms between the parental lines.

This combined approach ensures adequate depth of sequence coverage (using cheaper, short-insert technologies) to make contigs, in addition to smaller numbers of long reads (using more expensive long-insert or long-read technologies) to enable assembly of these contigs into scaffolds.

Finally, the depth of sequence coverage obtained using next-generation sequencing (approximately 70X for this project) means that it is likely that a near-complete Wolbachia genome sequence could be obtained and assembled from a single sequencing run even if far more substantial host contamination were present.

Following assembly, chloroplast contigs were merged using the minimus2 application of the AMOS 3.1.0 assembly package [ 26] running default parameters and for the titration of depths of sequence coverage, SeqMan (LazerGene) using a match size of 5, a minimum match percentage of 95 and a minimum sequence length of 1000.

The first data set was used to test performance as a function of sequencing coverage.

For each position in the CDS, the number of hits overlapping this position were counted and used as a measure of sequencing coverage.