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Most orthology determination methods make use of sequence comparisons: the amino acid sequences of all proteins in two species are compared with each other, and the two sequences that are most similar are considered to be orthologs.
Because of the extent of sequence conservation within the reverse transcriptase protein, the use of sequence comparisons of reverse transcriptase domains I and VII has been proposed to group retrotransposons into a family when the amino acid identity with other members is ≥90% and into a superfamily when their identity is ≥75% [ 16].
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The use of sequence comparison methods has been remarkably growing in recent years, in response to the data expansion of genomic research.
Several studies using methods of sequence comparison agree that functional conservation is common if two proteins possess more than 50% sequence identity [26] [30].
GenBank accession numbers of reference strains used for sequence comparisons are available at www.pcornastudygroup.com, some of which are indicated in the trees.
In addition, distinct regions of the genome sequences of other species were used for sequence comparisons.
We use peptide sequences as the basis of sequence comparison as they have a number of advantages over using nucleotide sequences [ 21].
In addition to the newly determined sequences, published full-genome sequences of AKAV and Oropouche virus (OROV) from the National Center for Biotechnology Information reference genome database (www.ncbi.nlm.nih.gov/sites/genome) were used for sequence comparisons and the reconstruction of phylogenetic relationships.
The use of DNA sequence comparisons for poor-quality sequences has the advantage that frame-shift artefacts arising from sequencing errors scarcely disturb the performed alignment.
The hexaploid T. aestivum was used as the primary basis of sequence comparison since the data sets available for T. aestivum are far larger than those for other Triticum species.
The library provided here (usm.js) advances that work by producing exact measure of similar length (Equations 3-5), and weaving its use in Equation 6 with MapReduce parallelization of sequence comparison.
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