The approximate quantification of volatiles collected from the headspace were calculated from GC peak areas, by comparison with the peak area of the 1,2-dichlorobenzene standard, using a response factor of 1.
The semi-quantification of semi-volatile compounds was calculated from the GC peak areas, by comparing with the peak area of the 3-chlorophenol standard, using a response factor of 1.
Calibration curves of each analyte were constructed using relative response factor against concentration of analyte from gas chromatography signals of prepared mixtures of known composition and used for estimating the percentage of each analyte in the liquid products.
The measured optical density was recalculated to biomass concentration using a response factor of 0.62, determined from measured final biomass dry weight.
We therefore examined the use of a universal response factor based on the average of seven compounds.
Briefly, each SVOC was quantified by comparing the integrated ion current response of the target ion to that of the internal standard using the average response factor of the target analyte generated from a standard calibration curve.
Glucosinolates were quantified by HPLC-UV using the desulfoglucosinolate method [ 9] and BGLS amounts were calculated relative to sinigrin using a relative response factor of 0.8 [ 10].
This present study was undertaken to find optimum conditions of pH, temperature and, period of incubation for the pectinolytic activity of Kluyveromyces wickerhamii isolated from rotting fruits and to assess the effect of these factors by use of response surface methodology (RS M.
This article highlights the use of response surface methodology by designing a three-factor five level central composite rotatable design matrix with full replication for planning, conduction, execution, and development of empirical relationship.
In these optimizations, the initial velocity of transformation was used as the response factor.
