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In the case of stochastic simulators, which produce a random output for a given set of model inputs, repeated evaluations are useful, supporting the use of replicate observations in the experimental design.
Our results demonstrate that the use of replicate naive populations can be a useful tool to differentiate between host and parasite adaptation in complex and dynamic fitness landscapes.
Several studies have found the use of replicate control samples such as UHRR to be a useful standard in microarray experiments, suitable for monitoring expression consistency within and across a variety of genome-wide expression platforms [ 29- 32].
First, the use of replicate sequencing increases costs and necessitates additional input nucleic acid amounts.
The use of replicate experiments is clearly essential in order to ascertain true expression change.
Staff performance was observed regularly and evaluated through the use of replicate measurements of all anthropometric data.
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SOSR makes use of replicates to estimate the second-order statistics, i.e. the autocovariance pattern of the noise.
The first involves the use of replicates at the different experimental points, while the second is a nonreplicated method.
Further, the use of replicates during analysis and the downstream confirmation of results using a supplemental methodology are important but frequently neglected aspects of experimental design.
Although some genetic variation exists within each landrace, the repetition of the drought experiment in 2 different years and the use of replicates of each landrace to obtain physiological and gene expression data produced consistent landrace specific data.
Thus, while vaccination with replication-restricted VACV recombinants is highly desirable for safety reasons, there might be an advantage in the use of replicating vectors eliciting prolonged immune responses.
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