Sentence examples for use of random primers from inspiring English sources

HRSV and HMPV RNA was extracted using Qiagen QIAamp viral RNA mini kit and reverse transcribed using Qiagen A3500 reverse transcription system, with extended elongation of 55 minutes and use of random primers.

Reverse transcription was performed by use of random primers.

We prefer the use of random primers to cover also non-polyadenylated transcripts in the libraries.

It was hoped that the use of random primers would remove a potential 3' bias in the ODT preparation.

A second (and any subsequent) round requires the use of random primers in reverse transcribing the output aRNA from the preceding round.

We foresee this protocol being practicable for unknown positive sense polyadenylated viruses through use of random primers and, where appropriate, an oligo-dT primer.

Priming with oligo dT reduced PCR yields and created 3' and sequence-specific biases compared with the use of random primer [ 13, 14, 25].

As mentioned above, the reason why bias is introduced is mainly due to the using of random primers and RNA degradation, on which our model is based.

KCNQ1OT1 strand specific cDNA was created in the same way except B-ACTIN reverse and KCNQ1OT1 reverse primers were used instead of random primers.

cDNA was prepared using the two-step TaqMan reverse transcription reagent (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions, except that a primer specific for c-kit RNA (KIT 2961R, 5′-TTCCTGGAGGGGTGACCCAAACACT-3′) was used instead of random primers.

First-strand of cDNA was synthesized from around 1 µg of DNA-free RNA using a set of random primers and M-MLV Reverse transcriptase (Invitrogen) following the supplier's instructions.