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Nickel ion-catalyzed peptide bond hydrolysis is an effective chemical cleavage technique under denaturing conditions that preclude the use of proteases.
For example, Bah reported the use of proteases to hydrolyse deer, sheep, pig and cattle red blood cell fractions to successfully obtain antimicrobial peptides (Bah et al. 2016).
This method consists of sequential incubation of mouse tibialis anterior muscles in latrunculin B, high ionic strength salt solution, and DNase I and avoids use of proteases or detergents that degrade the ECM.
Each new crystal was analyzed using mass spectrometry to ensure that the success of the method could be attributed directly to the use of proteases.
Additionally, the use of proteases from kiwi fruit, 291) bromelain from pineapple, and ficin from figs(292) has been reported.
Such a wide use of proteases in the industrial field shows their importance especially that they represent around 60% of the total enzyme market [ 3].
Similar(48)
Its clinical investigators launched the use of protease inhibitors in infected patients.
The Hong Kong Hospital Authority also said today that it was experimenting with alternative treatments for SARS for a few patients, including the use of protease inhibitors.
In the U.S., where AIDS deaths have been reduced dramatically by the use of protease inhibitors and other drugs, a dangerous sense of complacency seems to have settled in.
The prophylactic use of protease inhibitors in patients undergoing ERCP is still controversial.
The condition of use of protease and folate conjugase was evaluated, besides alternative treatments without enzyme use.
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