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Our use of pooled samples enabled us to test the different comparison groups against a large number of biological outcomes on trophoblast cells which would not have been possible if we had tested multiple samples from each of the studied groups.
One of the weaknesses in our present study is the use of pooled samples for initially global miRNA screening since results generated from pooled samples might yield some inaccurate information and missing useful information due to individual difference, and therefore decreases the specificity of the test.
There is a controversy about the use of pooled samples in the literature.
The limitations of this study include the use of pooled samples for discovery and the relatively small sample sizes.
As the use of pooled samples does not allow for investigations of linkage disequilibrium, we cannot ascertain the degree or distance of linkage between these regions of divergence.
To rule out any bias generated by the use of pooled samples, western blotting was also performed on individual cerebella resolved by SDS-PAGE.
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First, we studied the recovery of survivin levels using serial dilution of pooled samples with known concentrations of survivin (pool A).
Unfortunately, the infection rate of S. (Neophlebotomus) gemmea could not be determined since we used the DNA of pooled samples.
For example, several independent rare risk variants with implied functional roles were recently associated with inflammatory bowel disease using next-generation sequencing of pooled samples (Rivas et al., 2011), a design similar to that of Nejentsev et al. (2009) who undertook the first such study and whose data we re-examine here.
A serial dilution of pooled samples was used to create a standard curve.
Treating genotypes as quantitative values allows the same analysis framework to be used for pooled samples of DNA from multiple individuals.
More suggestions(15)
use of vocal samples
use of 'available samples
use of pooled cultures
use of free samples
use of limited samples
use of strange samples
use of different samples
use of pooled soils
use of human samples
use of pooled predictions
use of pooled embryos
use of pooled funds
use of unlicensed samples
use of archived samples
use of consolidated samples
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