Sentence examples for use of neural stem from inspiring English sources
Researchers say the finding places important restrictions on the potential therapeutic use of neural stem cells.
This lack of knowledge is a major barrier holding back the discovery of therapeutic uses of neural stem cells.
In addition, neural stem cells and neural progenitors have been isolated from human brain using genetic promoter-reporters of neural stem cell markers [24], [25].
Such a growth characteristic was first demonstrated in brain tumours, in which application of a culture condition used to support cultivation of neural stem cells led to the successful isolation of 3D neurospheres bearing CSC properties from human brain tumours (Hemmati et al, 2003; Singh et al, 2003).
Neuronal differentiation was then induced by subculture of neural stem cells using 0.05% Trypsin/EDTA in P-ACM for 14 days.
This assay has previously been used for the in vitro study of neural stem cell self-renewal [ 19] and relies on the observation that neurosphere formation is initiated clonally by neural stem cells [ 5].
Here we used various treatments to establish efficient cultures of neural stem cells from different areas of the adult rodent and monkey central nervous systems.
It can be expected that future studies will clarify the specific neurodevelopmental roles of these proteoglycans and their associated ligands in stem cell populations, and how these interactions can be used to advantage in the therapeutic application of neural stem cells for the treatment of injury, degeneration and aging in the CNS.
The formation of new NS after each successive passage has been widely used as a quantitative measure of the self-renewing activity of neural stem cells.
Similarly, they used Nestin-Cre and GFAP-Cre mice to demonstrate that mutation of neural stem cells or astrocytes can produce high-grade glioma in mice (Fig. 1).
We used the culture conditions that favor neural stem cell growth, previously established for isolation of neural stem cells as neurospheres [26].
