Sentence examples for use of negative controls from inspiring English sources

β-values were calculated by subtracting background with use of negative controls on the array and taking the ratio of the methylated signal intensity to the sum of both methylated and unmethylated signals.

In all cases, the use of negative controls is critical (2, 3 ).

Precautions such as the use of negative controls (blanks) and filter tips for PCR reactions are advised.

In purely experimental studies, the use of negative controls such as the blank or mock conditions is absolutely necessary.

The absence of ALDH1 expression (ALDH1neg) was also evaluated by the use of negative controls, at measurements of 60 and higher (range: 60 90, median: 70 ± 0.30).

For this study, we use two methods of quality control for genotyping: the use of negative controls and genotyping of replicate samples (at least 5% of total).

For biomarker analysis, the use of negative control is important, although it is also possible to monitor temporal variations of a biomarker response in only one sampling site.

Further, the use of negative control SEPs also accounts for the nonhomogenous delivery of the nanoparticles as well as the variability of the working distances between the optical device and the sample (Garai et al. 2015).

Demonstrating specificity through the use of negative control peptides is essential in this assay.

Although the original study considered a variety of chromosome abnormalities, we proceed as in the report by Rosenbaum (2), who focused on these particular outcomes to illustrate the use of negative control outcomes to detect the presence of unobserved confounding.

We also used the same number of negative controls as that of positive samples (Additional file 1: Table S1).