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This suggests that while the rbcL locus may be quantitatively useful, the simultaneous use of multiple loci may improve quantitative measurement of pollen abundance.
Nevertheless, the use of multiple loci enables us to observe the species clusters even in the presence of conflicting signals.
Moreover, while any one locus can provide a misleading estimate of population structure, our use of multiple loci and different analytical methods allowed us to estimate species limits while taking into account the idiosyncrasies of each data set.
In regards to the 'gene tree versus species tree' issue, it is generally accepted that the use of multiple loci would in principle provide a more complete picture of the divergence history for a group of closely related species [1].
These methods to identify distinct evolutionary lineages rely on the use of multiple loci and do not employ any kind of time calibration for the species delimitation tests.
The use of multiple loci for strain identification is preferred [ 23, 25] as a single locus cannot differentiate all the strains.
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The use of multiple locus genetic data appears thus as a powerful tool for recognizing species boundaries.
If the strains are pathogenic Y. enterocolitica, they are typed by use of multiple-locus variable-number tandem repeat analysis (MLVA), using multicolor capillary electrophoresis (5 ).
This study adds to a growing list of studies that support predictions of Haldane's Rule using cline analysis of multiple loci of differing inheritance modes.
This study adds to a growing list of studies that support predictions of Haldane's Rule using cline analysis of multiple loci of differing inheritance modes, although alternate hypotheses like selection on different mtDNA types cannot be ruled out.
The generation and comparison of benchmark genomes from all Cryptosporidium species and genotypes would help establish more definitive taxonomy than is currently possible based on the analysis of only a tiny proportion of the genome using Sanger sequencing even of multiple loci [ 19].
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