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Thus, these parameters reflect the concurrent use of multiple acceptors.
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The resulting assembly of multiple acceptors surrounding a donor permits this construct to exhibit high resonance energy transfer efficiency (∼64.8%).
The results from the Clade IC dominated reactor indicated that either organisms in this clade can simultaneously use multiple electron acceptors under micro-aerobic conditions, or that the use of multiple electron acceptors by Clade IC is due to significant microdiversity within the Accumulibacter clades defined using the ppk1 gene.
The use of multiple immunogens.
The objective of this study was to experimentally test the generality of using the sum of individual donor-acceptor FRET transfer rates (kD→A) to predict the ensemble FRET efficiency of a complex of a donor with multiple acceptors (equation 2).
Thus, the use of FRET as a tool for measuring protein-protein interactions inside living cells requires an understanding of how FRET changes with multiple acceptors.
The possibility of interaction between one donor and multiple acceptors is significant within the crowded environs of the macromolecular machine.
Investigations into such approaches and the use of other acceptors are underway.
It is known that splice sites mutations may disrupt protein function by diverse mechanisms such as exon skipping or the use of cryptic acceptor sites, presenting even multiple splice outcomes for a mutation in a given splice site [ Takahara et al., 2002].
These simple experimental systems have been used to explore general questions of proton uptake by proteins, such as the functional value of an initial proton acceptor, whether an initial acceptor must be surface-exposed, which side chains will function as initial proton acceptors and whether multiple acceptors can speed proton uptake.
We find that the amount of energy transfer observed in constructs having multiple acceptors is significantly greater than the FRET efficiency predicted from the sum of the individual donor to acceptor transfer rates.
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