Sentence examples for use of multilocus sequencing from inspiring English sources

New Aspergillus species have recently been described with the use of multilocus sequencing in refractory cases of invasive aspergillosis.

Use of multilocus sequence typing may help identify new strains in at-risk populations.

The use of multilocus sequence data requires different statistical procedures, which become more and more sophisticated.

The widespread use of multilocus sequence typing (MLST) [ 17] for epidemiological purposes provides the sequences of seven house-keeping gene fragments from thousands of isolates of several bacterial pathogens.

Intriguingly, similar lineages consisting of 16S type II isolates from cats were observed in other genotyping studies involving the use of multilocus sequence typing (MLST) (14 ) and multiple locus variable number tandem repeat analysis (MLVA) (15 ).

While the use of multilocus sequence typing (MLST) incorporating loci such as hsp65 and secA undoubtedly would aid in the more accurate identification of isolates, the current economics of veterinary diagnostic testing rule against routine use of MLST in our laboratory.

Here we used phylogenetic and coalescent-based analyses of multilocus sequence data to investigate the origin of H. heurippa.

Here, we used the gene-based, recently developed technique of multilocus sequence typing (MLST) (available from www.mlst.net) to describe the characteristics of these nasal S. aureus strains from farmers and controls and the relationships between strains, and investigated their possible animal origin by comparing them with strains isolated from infected pigs from the same geographic area.

Amplification of multilocus sequence typing (MLST) gene fragments was carried out using primers and protocols described previously [10].

Relationships among isolates were inferred from Rep-PCR genotypes by using published methods that maximize the correspondence of such inferences to the standard of multilocus sequence typing (30 ).

All the strains had previously been characterized by means of multilocus sequence typing (MLST).